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KMID : 1234420080360030209
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Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 3 p.209 ~ p.214
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Characterization of Viable But Nonculturable Condition of Escherichia coli Induced with Copper
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Ku Hyung-Keun
Park Sang-Ryoul
Kim Sook-Kyung
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Abstract
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VBNC (Viable but nonculturable) state is an adaptive response of cells in adverse environments, which lead cell not grow on routine nutrient agar. In this study, we induced VBNC in Escherichia coli using copper and verify the characterization of it. After treatment of copper, we didn``t detect any cells via plate cultivation, namely, colony forming unit (CFU) was zero. However, we identified the existence of VBNC by staining live cells with Live/Dead BacLight bacterial viability kit and counting them through flow cytometry. Then we isolated genomic DNA and RNA from VBNC-induced cells and analyzed the stability of them. Degradation of RNA is more severe than that of DNA and RNA is degraded as specific fragments. In addition, we showed the morphology of VBNC cell by Bio-Transmission Electron Microscope (Bio-TEM). VBNC cell showed impaired periplasmic space and inner and outer membrane were separated and the amount of cytosol were significantly decreased.
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KEYWORD
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VBNC, Escherichia coli, copper, Bio-TEM
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